[KO]EGFR Rabbit mAb

Key Features

货号	RABZ027
别名Alternative Name	EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c- ErbB-1; Receptor tyrosine-protein kinase erbB-1
应用	WB, IHC-P, ICC/IF, FC, IP
反应种属	Human, Mouse, Rat
稀释比	WB-1:1000-1:5000; IHC-P-1:50-1:100; ICC/IF-1:200-1:500; FC-1:20; IP-1:20
SwissProt ID	P00533
宿主	Rabbit
偶联物	Unconjugated
修饰	Unmodified
基因 ID	1956
基因名	EGFR
亚型	IgG
克隆性	Monoclonal Antibody
克隆号	R03-6B1
分子量	Calculated MW: 134 kDa; Observed MW: 175 kDa
免疫原	A synthetic peptide of human EGFR
研究领域	Signal Transduction
背景信息	EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation.
纯化方式	Affinity Purified
形式	Liquid
存储缓冲液	Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
保存温度	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

图片

Western blot analysis of EGFR in HeLa(WT) and EGFR-Knockout HeLa(KO) using EGFR antibody.

Western blot analysis of EGFR in mouse liver lysates using EGFR antibody.

Western blot analysis of EGFR in Hela lysates using EGFR antibody.

Immunocytochemistry analysis of EGFR(green) in Hela using EGFR antibody,and DAPI(blue)

性能

纯化方式	Affinity Purified
形式	Liquid
存储缓冲液	Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
保存温度	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.