货号

RABZ016

别名Alternative Name

TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

应用

WB, IHC-P, ICC/IF, FC, IP

反应种属

Human

稀释比

WB-1:1000-1:5000; IHC-P-1:20-1:50; ICC/IF-1:50-1:100; FC-1:200-1:2000; IP-1:20

SwissProt ID

P04637

宿主

Rabbit

偶联物

Unconjugated

修饰

Unmodified

基因 ID

7157

基因名

TP53

亚型

IgG

克隆性

Monoclonal Antibody

克隆号

R07-5G9

分子量

Calculated MW: 44 kDa; Observed MW: 53 kDa

免疫原

A synthesized peptide derived from human p53

研究领域

Cell Biology

背景信息

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

纯化方式

Affinity Chromatography

形式

Liquid

存储缓冲液

Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存温度

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

纯化方式

Affinity Chromatography

形式

Liquid

存储缓冲液

Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存温度

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

IHC-P

1:20-1:50

WB

1:1000-1:5000

ICC/IF

1:50-1:100

FC

1:200-1:2000

IP

1:20