货号

RABZ018

别名Alternative Name

NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB

应用

WB, IHC-F, IHC-P, ICC/IF, IP

反应种属

Human, Mouse

稀释比

WB-1:1000-1:5000; IHC-P-1:1000-1:5000; ICC/IF-1:50-1:100; IP-1:20-1:50

SwissProt ID

Q04206

宿主

Rabbit

偶联物

Unconjugated

修饰

Unmodified

基因 ID

5970

基因名

RELA

亚型

IgG

克隆性

Monoclonal Antibody

克隆号

R09-3H2

分子量

Calculated MW: 60 kDa; Observed MW: 65 kDa

免疫原

A synthetic peptide of human NF-kB p65

研究领域

Cell Biology

背景信息

Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which   regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel, p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF- kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF- kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

纯化方式

Affinity Purified

形式

Liquid

存储缓冲液

Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

保存温度

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

纯化方式

Affinity Purified

形式

Liquid

存储缓冲液

Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

保存温度

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

IHC-P

1:1000-1:5000

WB

1:1000-1:5000

ICC/IF

1:50-1:100

IP

1:20-1:50