货号

RABZ026

别名Alternative Name

CCND1; BCL1; D11S287E; PRAD1; U21B31; Cyclin D1

应用

WB, IHC-P, ICC/IF, IP

反应种属

Human, Mouse, Rat

稀释比

WB-1:1000-1:2000; IHC-1:100-1:200; IF-1:50-1:200; IP-1:50

SwissProt ID

P24385

宿主

Rabbit

偶联物

Unconjugated

修饰

Unmodified

基因 ID

595

基因名

CCND1

亚型

IgG

克隆性

Monoclonal Antibody

克隆号

R09-7J5

分子量

Calculated MW: 34 kDa; Observed MW: 34 kDa

免疫原

A synthesized peptide derived from human Cyclin D1

研究领域

Cell Biology

背景信息

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.  Mutations,  amplification  and  overexpression  of this  gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

纯化方式

Affinity Chromatography

形式

Liquid

存储缓冲液

1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA

保存温度

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

纯化方式

Affinity Chromatography

形式

Liquid

存储缓冲液

1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA

保存温度

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

IHC

1:100-1:200

WB

1:1000-1:2000

IF

1:50-1:200

IP

1:50