货号

RABZ017

别名Alternative Name

AKT1/AKT2/AKT3; Pan-Akt

应用

WB, IHC-F, IHC-P, ICC/IF, FC, IP

反应种属

Human, Mouse, Rat

稀释比

WB-1:1000-1:20000;  IHC-1:100-1:2000;  ICC/IF-1:50-1:100;  

FC-1:200-1:500;  IP-1:50- 1:100

SwissProt ID

P31749/P31751/Q9Y243

宿主

Rabbit

偶联物

Unconjugated

修饰

Unmodified

基因 ID

207/208/10000

基因名

AKT1/AKT2/AKT3

亚型

IgG

克隆性

Monoclonal Antibody

克隆号

R04-8A4

分子量

Calculated MW: 56 kDa;  Observed MW: 56 kDa

免疫原

Recombinant protein of human AKT1/2/3

研究领域

Signal Transduction

背景信息

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase.  Akt  is  activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

纯化方式

Affinity Purified

形式

Liquid

存储缓冲液

1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA

保存温度

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

纯化方式

Affinity Purified

形式

Liquid

存储缓冲液

1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA

保存温度

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

IHC

1:100-1:2000

WB

1:1000-1:20000

ICC/IF

1:50-1:100

FC

1:200-1:500

IP

1:50- 1:100