蛋白质全称

His标签

别名Alternative Name

6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED

免疫原

KLH偶联的HHHHHH (6* His)合成肽

偶联

辣根过氧化物酶

纯化方式

亲和纯化

背景

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

应用

物种

稀释

阳性样品

WB免疫印迹

Species independent

1: 2000-1: 5000

His-Tag融合蛋白

孵化条件

封闭后, 将膜与HRP标记的抗His Tag 抗体(RABS078)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.