蛋白质全称

MYC标签

别名Alternative Name

c-myc, Myc, MYC, Myc Epitope Tag, Myc tag, MYC tag, EQKLISEEDL, tag, Tag, TAG

免疫原

KLH偶联的EQKLISEEDL合成肽

抗体亚型

IgG, κ

克隆号

SB226

纯化方式

亲和纯化

背景

The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal.

储存条件

在-20℃下储存一年, 避免反复冻融.

储存缓冲液

含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

应用

物种

稀释

阳性样品

WB免疫印迹

Species independent

1: 500-1: 1000

MYC标签融合蛋白

ICC/IF免疫细胞化学/免疫荧光

Species independent

1: 500-1: 1000

expi293细胞