蛋白质全称

HA标签

别名Alternative Name

HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

免疫原

KLH偶联的YPYDVPDYA合成肽

偶联

辣根过氧化物酶

纯化方式

亲和纯化

背景

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

应用

物种

稀释

阳性样品

WB免疫印迹

Species independent

1: 1000-1: 2000

HA标签融合蛋白

孵化条件

封闭后, 将膜与HRP标记的抗HA tag 抗体(RABS077)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.