蛋白质全称

GST标签融合蛋白

别名Alternative Name

Glutathione S-Transferase, GST, gst tag

免疫原

GST重组蛋白

偶联

辣根过氧化物酶

纯化方式

亲和纯化

背景

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

应用

物种

稀释

阳性样品

WB免疫印迹

Species independent

1: 1000-1: 3000

GST-Tag 融合蛋白

孵化条件

封闭后, 将膜与HRP标记的抗GST Tag 抗体(RABS074)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.