RFP Recombinant Rabbit MAB

Key features and details

产品说明书

货号:RABS069-100ul

价格:
品牌: Lifeman
货期: 3-5个工作日
规格:
数量:
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Protein full name

Red fluorescent protein drFP583

Synonyms/Alternative Name

Red fluorescent protein, Red fluorescent protein eqFP611, RFPTag, RFP-Tag, RFP Tag

Immunogen

Recombinant protein corresponding to RFP

Uniprot ID

Q9U6Y8

Isotype

IgG, κ

Purity

Affinity purification

Background

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein  interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.

Alternate Text
ICC analysis of RFP red.

Sample: Expi293, 4% PFA 20min.

Blocking buffer: 3% BSA in PBS, RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) , 1: 300, RT, 1h.

Storage

Store at -20 for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

Applications

Species

Dilution

Positive Tissue

ICC/IF

Species independent

1: 500-1: 2000

Expi293

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