GST Tag Recombinant Rabbit MAB

Key features and details

产品说明书

货号:RABS075-100ul

价格:
品牌: Lifeman
货期: 3-5个工作日
规格:
数量:
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蛋白质全称

GST标签融合蛋白

别名Alternative Name

Glutathione S-Transferase, GST, gst tag

免疫原

重组GST蛋白

抗体亚型

IgG, κ

克隆号

SB79

纯化方式

亲和纯化

背景

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Alternate Text
WB检测GST Tag(货号 RABS075).

样品: 经RIPA裂解液处理的蛋白质.

封闭: 3%脱脂牛奶溶于TBST溶液, 室温孵育1小时.

—抗: 1: 1000稀释, 4℃ 孵育过夜.

二抗: HRP标记山羊抗兔IgG (H+L) , 1: 5000稀释, 室温孵育1小时.

储存条件

-20℃下储存一年, 避免反复冻融.

储存缓冲液

0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

应用

物种

稀释

阳性样品

WB免疫印迹

Species independent

1: 1000-1: 3000

转染的HEK-293F细胞

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