蛋白质全称

Flag标签

别名Alternative Name

DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG

免疫原

KLH偶联的DYKDDDDK合成肽

抗体亚型

IgG, κ

克隆号

SB75

纯化方式

亲和纯化

背景

The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

储存条件

在-20℃下储存一年, 避免反复冻融.

储存缓冲液

含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

应用

物种

稀释

阳性样品

WB免疫印迹

Species independent

1: 2000-1: 5000

转染的HEK-293F细胞

ICC/IF免疫细胞化学/免疫荧光

Species independent

1: 500-1: 1000

转染的HEK-293F细胞