蛋白质全称

Flag标签

别名Alternative Name

DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG

免疫原

KLH偶联的DYKDDDDK合成肽

偶联

辣根过氧化物酶

纯化方式

亲和纯化

背景

The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

应用

物种

稀释

阳性样品

WB免疫印迹

Species independent

1: 2000-1: 5000

Flag-Tag 融合蛋白

孵化条件

封闭后, 将膜与HRP标记的抗FLAG Tag 抗体(RABS072)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.