Key features and details

  • Target:DYKDDDDK Tag; Flag; FLAG
  • Applications:WB, IP
  • Host Species:Mouse
  • Reactivity:Species-independent
产品说明书

货号:MABZ007-100ul

价格:
品牌: Lifeman
货期: 7天
规格:
数量:
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Gene name

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Alternative Names

DYKDDDDK Tag; Flag; FLAG

UniProt ID

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Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged proteins biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

Alternate Text
Western blot analysis of HA Tag (3C6) in CHO-K1 (D) or CHO-K1 transfected with different Flag fusion proteins (A, B, C), using Flagtag antibody.
Alternate Text
Western blot analysis of Flag Tag (10E1) in CHO-K1 cells transfected with Flag GADD45α (C and D), 3*Flag GADD45α (A and B) as indicated, using Flag tag antibody (left) and GADD45α antibody (right) as loading control.

组成

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

纯化工艺

Protein A

储存

-20°C

推荐稀释比 

WB:

1:1000-10000

IP:

1:500

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