Catalog No.

ZS204

Brand

Lifeman

Alternative Name

Goat Anti-Rabbit IgG Heavy & Light Chains (Alexa Fluor 555 conjugate)

Application

IHC-P,ICC/IF,FC,ELISA

Reactivity

Rabbit

Dilution Ratio

IHC-P: 1:100–1:1000
ICC/IF: 1:100–1:1000
FC: 1:1000–1:4000      ELISA-Use at an assay dependent concentration

Host

Goat

Clonality

Polyclonal Antibody

Immunogen

Rabbit IgG

Research Area

Immunology

Background

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent.The average molecular weight is reported to be about 150 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Conjugate

Alexa Fluor 555
(Excitation: 553 nm, Emission: 568 nm)

Purification

Affinity Purified

Form

Liquid

Storage Buffer

Liquid  in  0.01M  Phosphate  Buffered  Saline,  pH  7.2,  containing  1%  BSA,  50% glycerol, 0.05% Sodium Azide

Storage Temperature

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Dilution Ratio

IHC-P: 1:100–1:1000
ICC/IF: 1:100–1:1000
FC: 1:1000–1:4000      ELISA-Use at an assay dependent concentration