Phospho-NF-KB p65 (Thr254) Rabbit pAb

Key features and details

  • Target:NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB
  • Applications:WB, IHC-P, ICC/IF
  • Host Species:Rabbit
  • MW:60kD (Calculated) ;65kD (Observed)
  • Reactivity:Human,Mouse,Rat
产品说明书

货号:PABZ002-100ul

价格:
品牌: Lifeman
货期: 7天
规格:
数量:
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Gene name

RELA

Alternative Names

NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB

UniProt ID

Q04206

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

Alternate Text
Western blot analysis of Phospho-NF-KB p65 (Thr254) in 3T3 lysates untreated or treated with TNFα using NFκBp65 (Phospho-Thr254) antibody
Alternate Text
Immunofluorescence analysis of Phospho-NF-KB p65 (Thr254) in Hela using NFκBp65(Phospho-Thr254) antibody.
Alternate Text
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using NFκBp65 (Phospho-Thr254) antibody (left) or the same antibody preincubated with blocking peptide (right).Highpressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

组成

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

纯化工艺

Protein A

储存

-20°C

推荐稀释比 

WB:

1:500-1000

IHC:

1:50-100

IF:

1:50-200

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